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AUTHORS: Gábor Nagy, Department of Animal Nutrition, Kaposvár University; Ágnes Csivincsik, Institute of Diagnostic Imaging and Radiation Oncology, Kaposvár University; Attila Zsolnai, Research Institute for Animal Breeding, Nutrition and Meat Science; László Sugár*, Department of Game Biology and Ethology, Kaposvár University
ABSTRACT: The anthelmintic resistance of gastrointestinal nematodes is one of the most important economic risk factors in grazing ruminant systems, all over the world [1]. Underdosing (i.e. a subtherapeutic dose rate) is one of the most important factors contributing to the development of AR [2]. In this study, we aimed to determine the presence of BZ resistance in the generalist worm, Haemonchus contortus in a microregion, where the resources partly shared by sheep, free-ranging red and roe deer and in a farmed red deer herd (Fig. 1). In this survey, we used a RFLP-PCR method [3]; and the nomenclature of genotype and allele frequency, we followed detailed by Pierce [4], and their proportions were determined with 95% confidence interval (CI95%). For comparison of genotypes and alleles in the ruminant populations, the chi-square test was performed with Bonferroni correction using R statistical software i386 3.3.0 version.